Under conditions of nutrient deprivation, the Gram positive soil
bacterium Bacillus subtilis can abandon vegetative
growth and form a dormant, environmentally-resistant spore instead. The
decision to either divide or sporulate is controlled by a large and
complex genetic regulatory network integrating various environmental,
cell-cycle, and metabolic signals. Although sporulation in
B. subtilis is one of the best-understood model systems for
prokaryotic development, very little quantitative data on kinetic
parameters and molecular concentrations are available. A qualitative
simulation method is used to model the sporulation network and simulate
the response of the cell to nutrient deprivation. Using this method, we
have been able to reproduce essential features of the choice between
vegetative growth and sporulation, in particular the role played by
competing positive and negative feedback loops.